Information-enriched characterization of cell populations. Morphological info from illustrations or photos can insert towards the richness of flow cytometry facts. One example is, the figure demonstrates an in any other case typical apoptosis assay utilizing Annexin V and PI, including mobile imaging to characterize cells in Each and every populace to reveal morphologically distinctive features. These insights could not are already acquired from multiplex staining by itself.
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(B) RAW 264.7 macrophages are often annotated inaccurately with respect to particle rely because of their complexity and noncircular borders. The number of singlets available for Assessment improves after the user trains and employs a more correct product.
Learn new insights into your experimental facts by visualizing populations using blended fluorescence and morphology knowledge. Our software package interface wizards allow downsampling, concatenation (merged data files) and dimensionality applications which can be built into the software for an intuitive, streamlined details Investigation practical experience.
"An image isn't really pretty worthy of 1000 dots, but it definitely really helps to see photos. It is excellent to check out images that correlate with staining. As an example, AnnexinV-constructive cells which have been morphologically various than healthier cells, or CD14+ monocytes that happen to be bigger and more textured than CD3+/CD4+/CD14- lymphocytes.
Right here, a qualified consumer has gated singlets confidently. Right after evaluating the manual singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate is made up of greater than four% aggregates.
Pair your Attune flow cytometer having an autosampler for improved workflow efficiency, and include robotic integration for max throughput.
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Early signs of this contamination experienced initially been dismissed as debris. Backgating to discover new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also permits you to use morphological characteristics to discover attention-grabbing subpopulations that would not be apparent from flow cytometry info on your own.
Possibly most of all, these situations comprise cells of clearly diverse phenotypes which may lead to incorrect conclusions relating to double optimistic events (specifically in uncommon populations).
Sound waves allow exact cell alignment, resulting in Remarkable data quality and ~10X more rapidly sample flow costs.
We Beforehand demonstrated the power of imaging Car or truck-T/Ramos cell interactions. Let’s check out just the population of biggest interest, the double constructive functions, To find out more. We are able to now use prolonged morphological parameters (here, circularity and skewness of depth) to even more study the features of those populations and refine gating on these activities, expanding facts robustness.
Leverage the strength of Highly get more info developed technologies and innovation to accelerate your discoveries. Have faith in within the Attune Flow Cytometer household to provide the reliability, pace, and accuracy that set your work aside.
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Gating on these extended parameters helps you to immediately and precisely recognize populations of curiosity and confirm gating strategy with little if any manual review. The Attune application provides access to point out-of-the-artwork device Understanding Assessment tools in just an expertly made person interface, making it exceptionally simple to operate.
Since the cell line had Earlier been analyzed about the Attune CytPix Flow Cytometer, the researchers went back to the pictures and had been ready to doc the microbial infection no less than five days previously.
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In this article, we exhibit that by gating procedures making use of picture-dependent quantitative parameters can distinguish interacting cells from coincident events to a lot more correctly review interacting cells.